Telomouse-a mouse model with human-length telomeres generated by a single amino acid change in RTEL1.

Smoom R, May CL, Ortiz V, Tigue M, Kolev HM, Rowe M, Reizel Y, Morgan A, Egyes N, Lichtental D, Skordalakes E, Kaestner KH, Tzfati Y.

Nat Commun. 2023 Oct 23;14(1):6708. doi: 10.1038/s41467-023-42534-6.

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The many faces of the helicase RTEL1 at telomeres and beyond.

Hourvitz N, Awad A, Tzfati Y.

Trends Cell Biol. 2023 Jul 31:S0962-8924(23)00135-6. doi: 10.1016/j.tcb.2023.07.002.

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Accelerated replicative senescence of ataxia-telangiectasia skin fibroblasts is retained at physiologic oxygen levels, with unique and common transcriptional patterns.

Haj M, Levon A, Frey Y, Hourvitz N, Campisi J, Tzfati Y, Elkon R, Ziv Y, Shiloh Y.

Aging Cell. 2023 Aug;22(8):e13869. doi: 10.1111/acel.13869. Epub 2023 May 30.

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Functional Interactions of Kluyveromyces lactis Telomerase Reverse Transcriptase with the Three-Way Junction and the Template Domains of Telomerase RNA.

Al-Shareef W, Brown Y, Bryan C, Shuvaeva E, Bsoul S, Greenman R, Kabaha MM, Ulyanov NB, Skordalakes E, Tzfati Y.

Int J Mol Sci. 2022 Sep 15;23(18):10757. doi: 10.3390/ijms231810757.

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Leukocyte Telomere Length Correlates with Extended Female Fertility.

Michaeli J, Smoom R, Serruya N, El Ayoubi H, Rotshenker-Olshinka K, Srebnik N, Michaeli O, Eldar-Geva T, & Tzfati Y.

Cells. 2022 Feb 2;11(3):513; doi: 10.3390/cells11030513.

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Inherited human Apollo deficiency causes severe bone marrow failure and developmental defects.

Kermasson L, Churikov D, Awad A, Smoom R, Lainey E, Touzot F, Audebert-Bellanger S, Haro S, Roger L, Costa E, Mouf M, Bottero AJ, Oleastro M, Abdo C, de Villartay JP, Geli V, Tzfati Y, Callebaut I, Danielian S, Soares G, Kannengiesser C, & Revy P.

Blood. 2022; doi: 10.1182/blood.2021010791.

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Telomerase, the recombination machinery and Rap1 play redundant roles in yeast telomere protection.

Kabaha M M & Tzfati Y. 

Current Genetics. 2020 Nov 6; doi: 10.1007/s00294-020-01125-4. 

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Full length RTEL1 is required for the elongation of the single-stranded telomeric overhang by telomerase.

Awad A, Glousker G, Lamm N, Tawil S, Hourvitz N, Smoom R, Revy P, & Tzfati Y.

Nucleic Acids Res. 2020 Jul 27;48(13):7239-7251.

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Identification of telomerase RNAs in species of the Yarrowia clade provides insights into the co-evolution of telomerase, telomeric repeats and telomere-binding proteins.

Červenák F, Juríková K, Devillers H, Kaffe B, Khatib A, Bonnell E, Sopkovičová M, Wellinger RJ, Nosek J, Tzfati Y, Neuvéglise C, Tomáška L.

Sci Rep. 2019. 16(1):13365.

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Trypanosoma brucei UMSBP2 is a single-stranded telomeric DNA binding protein essential for chromosome end protection.

Klebanov-Akopyan1 O, Mishra A, Glousker G, Tzfati Y, Shlomai J.

Nucleic Acids Res. 2018 Sep 6;46(15):7757-7771.

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Human RTEL1 stabilizes long G-overhangs allowing telomerase-dependent over-extension.

Porreca RM, et al.

Nucleic Acids Res. 2018 May 18;46(9):4533-45.

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Mutations in STN1 cause Coats plus syndrome and are associated with genomic and telomere defects.

Simon AJ, et al.

J Exp Med. 2016. 213(8), 1429–40.

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Unraveling the pathogenesis of Hoyeraal-Hreidarsson syndrome, a complex telomere biology disorder.

Glousker G, Touzot F, Revy P, Tzfati Y, Savage SA.

Br J Haematol. 2015 May 4;170(4):457-71.

 

Inherited mutations in the helicase RTEL1 cause telomere dysfunction and Hoyeraal-Hreidarsson syndrome.

Deng Z, Glousker G, Molczan A, Fox AJ, Lamm N, Dheekollu J, Weizman OE, Schertzer M, Wang Z, Vladimirova O, Schug J, Aker M, Londoño-Vallejo A, Kaestner KH, Lieberman PM, Tzfati Y.

Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3408-16.

 

Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo.

Cash DD, Cohen-Zontag O, Kim NK, Shefer K, Brown Y, Ulyanov NB, Tzfati Y, Feigon J.

Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):10970-5.

 

Identification of telomerase RNAs from filamentous fungi reveals conservation with vertebrates and yeasts.

Kuprys PV, Davis SM, Hauer TM, Meltser M, Tzfati Y, Kirk KE.

PLoS One. 2013;8(3):e58661.

 

The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family.

Gupta SK, Kolet L, Doniger T, Biswas VK, Unger R, Tzfati Y, Michaeli S.

FEBS Lett. 2013 May 2;587(9):1399-404.

 

TER structure, function and molecular mechanisms.

Tzfati Y, Chen J.

Book chapter in: Telomerases: Chemistry, Biology and Clinical Applications. Autexier C, Lue NF, editors. John Wiley & Sons, Inc.; 2012.

 

"Poisoning" yeast telomeres distinguishes between redundant telomere capping pathways.

Lamm N, Bsoul S, Kabaha MM, Tzfati Y.

Chromosoma. 2012 Dec;121(6):613-27.

 

Electron-transfer quenching of nucleic acid-functionalized CdSe/ZnS quantum dots by doxorubicin: a versatile system for the optical detection of DNA, aptamer-substrate complexes and telomerase activity.

Raichlin S, Sharon E, Freeman R, Tzfati Y, Willner I.

Biosens Bioelectron. 2011 Aug 15;26(12):4681-9.

 

DNAzyme-like activity of hemin-telomeric G-quadruplexes for the optical analysis of telomerase and its inhibitors.

Freeman R, Sharon E, Teller C, Henning A, Tzfati Y, Willner I.

Chembiochem. 2010 Nov 22;11(17):2362-7.

 

Optical, electrical and surface plasmon resonance methods for detecting telomerase activity.

Sharon E, Freeman R, Riskin M, Gil N, Tzfati Y, Willner I.

Anal Chem. 2010 Oct 15;82(20):8390-7.

 

Diminished telomeric 3' overhangs are associated with telomere dysfunction in Hoyeraal-Hreidarsson syndrome.

Lamm N, Ordan E, Shponkin R, Richler C, Aker M, Tzfati Y.

PLoS One. 2009 May 22;4(5):e5666.

 

Identification and comparative analysis of telomerase RNAs from Candida species reveal conservation of functional elements.

Gunisova S, Elboher E, Nosek J, Gorkovoy V, Brown Y, Lucier JF, Laterreur N, Wellinger RJ, Tzfati Y, Tomaska L.

RNA. 2009 Apr;15(4):546-59.

 

The 5' arm of Kluyveromyces lactis telomerase RNA is critical for telomerase function.

Kabaha MM, Zhitomirsky B, Schwartz I, Tzfati Y.

Mol Cell Biol. 2008 Mar;28(6):1875-82.

 

A critical three-way junction is conserved in budding yeast and vertebrate telomerase RNAs.

Brown Y, Abraham M, Pearl S, Kabaha MM, Elboher E, Tzfati Y.

Nucleic Acids Res. 2007;35(18):6280-9.

 

Pseudoknot structures with conserved base triples in telomerase RNAs of ciliates.

Ulyanov NB, Shefer K, James TL, Tzfati Y.

Nucleic Acids Res. 2007;35(18):6150-60.

 

Telomerase core components protect Candida telomeres from aberrant overhang accumulation.

Hsu M, McEachern MJ, Dandjinou AT, Tzfati Y, Orr E, Blackburn EH, Lue NF.

Proc Natl Acad Sci U S A. 2007 Jul 10;104(28):11682-7.

 

A triple helix within a pseudoknot is a conserved and essential element of telomerase RNA.

Shefer K, Brown Y, Gorkovoy V, Nussbaum T, Ulyanov NB, Tzfati Y.

Mol Cell Biol. 2007 Mar;27(6):2130-43.

 

A universal telomerase RNA core structure includes structured motifs required for binding the telomerase reverse transcriptase protein.

Lin J, Ly H, Hussain A, Abraham M, Pearl S, Tzfati Y, Parslow TG, Blackburn EH.

Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14713-8.

 

A template-proximal RNA paired element contributes to Saccharomyces cerevisiae telomerase activity.

Seto AG, Umansky K, Tzfati Y, Zaug AJ, Blackburn EH, Cech TR.

RNA. 2003 Nov;9(11):1323-32.

 

A novel pseudoknot element is essential for the action of a yeast telomerase.

Tzfati Y, Knight Z, Roy J, Blackburn EH.

Genes Dev. 2003 Jul 15;17(14):1779-88.

 

A bulged stem tethers Est1p to telomerase RNA in budding yeast.

Seto AG, Livengood AJ, Tzfati Y, Blackburn EH, Cech TR.

Genes Dev. 2002 Nov 1;16(21):2800-12.

 

Collaboration can work if inequality is recognized.

Tzfati Y.

Nature. 2002 Jun 13;417(6890):689.

 

Template boundary in a yeast telomerase specified by RNA structure.

Tzfati Y, Fulton TB, Roy J, Blackburn EH.

Science. 2000 May 5;288(5467):863-7.

 

Genomic organization and expression of the gene encoding the universal minicircle sequence binding protein.

Tzfati Y, Shlomai J.

Mol Biochem Parasitol. 1998 Jul 1;94(1):137-41.

 

A single-stranded DNA binding protein binds the origin of replication of the duplex kinetoplast DNA.

Avrahami D, Tzfati Y, Shlomai J.

Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10511-5.

 

Universal minicircle sequence binding protein, a CCHC-type zinc finger protein that binds the universal minicircle sequence of trypanosomatids. Purification and characterization.

Tzfati Y, Abeliovich H, Avrahami D, Shlomai J.

J Biol Chem. 1995 Sep 8;270(36):21339-45.

 

Primary products of break-induced recombination by Escherichia coli RecE pathway.

Silberstein Z, Tzfati Y, Cohen A.

J Bacteriol. 1995 Apr;177(7):1692-8.

 

A trypanosomal CCHC-type zinc finger protein which binds the conserved universal sequence of kinetoplast DNA minicircles: isolation and analysis of the complete cDNA from Crithidia fasciculata.

Abeliovich H, Tzfati Y, Shlomai J.

Mol Cell Biol. 1993 Dec;13(12):7766-73.

 

A single-stranded DNA-binding protein from Crithidia fasciculata recognizes the nucleotide sequence at the origin of replication of kinetoplast DNA minicircles.

Tzfati Y, Abeliovich H, Kapeller I, Shlomai J.

Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6891-5.